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. 2018 Jul 16;9:2739. doi: 10.1038/s41467-018-05157-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — CD32 is a marker of T-cell activation. a Flow cytometry dot plots showing co-expression of CD32 and markers of cell activation and proliferation in unstimulated (UN) PBMCs or those stimulated with IL-2, PHA/IL-2, αCD3/αCD28/IL-2, and IL-7/IL-2. A representative donor is shown. b Fold change of CD32 expression in CD4+ T cells unstimulated or stimulated with different conditions from uninfected donors. The cells were cultured in the presence of different stimuli for 72 h, and protein levels of the cell surface marker CD32 were evaluated by flow cytometry. c Percentage of Ki67+ cells after activation with different stimuli as in (a). d Upregulation of CD32 correlates with the expression of activation markers HLA-DR and CD69 after activation with the different stimuli. Relative contribution of HLA-DR (left panel) or CD69 (right panel) cells over the total population of CD32-expressing cells. e Individual data of HLA-DR cells (left panel) or CD69 (right panel) cells in the CD32 compartment. The gating strategy used to identify CD32+ cells is shown in Supplementary Fig. 1. All panels represent the mean ± SD of at least five different donors. Student’s t-test, *p < 0.05, **p < 0.005, ***p < 0.0005