Table 3.
Primers used in this work.
| Gene | Forward primer sequences (5′-3′) | Reverse primer sequences (5′-3′) |
|---|---|---|
| Real-time quantitative PCR | ||
| SCD1 | TACTCCGAGACAGACGCAGAC | GCCACAGCCAAGGATTCAC |
| ApoE | TGGCAGAGCTGGCTACATACA | TTGCGGACATCGTCAGAGTT |
| CPT-1α | GCTGTTGCCACGGGAGATAG | CTGCTCAGTGTCATCAAGGGTT |
| FABP1 | TGAACTCATCCAGAAAGGCAA | CCGTCACGCTGAACCACA |
| PEPCK | CCTGCTGCGAGTCCCTGA | TACTTCCTGACCTCCTCCACC |
| GS | CTTGAGGAGTGGGACGAGGA | GAGGTCAGACGGGCTTTGGT |
| LPL | TGTGTCCAAGTTCTCCCTGCG | CCAGCCATGTATCACAATGAAGC |
| FAS | TCGTCATGGCTATGAGGGAGAT | GTTGAGTGGCGTCACTGTGG |
| ME | CTTGCAGAGCAGGTGACAGATAA | GGCAGGAAGGAGTCGTAGTGG |
| 6GPD | CAGGGACGAGGAGGCTACTT | CACGTCTGACATGGACACTGG |
| SREBP1 | TGCTATCGGTTCTAACATGGCTAC | AGTGCTCAACAGTCAGATACAGTC |
| DGAT | CTGAGCCATCATTCCCATAAAG | GTTCACAAGTGGTGCCTGAGAC |
| GPAT | AATGAAACGCCAACCCTGC | GTGTAGTGCTCCTGAAATCTATCCT |
| ACO1 | CACTGGTAGATGCCTTTGACG | CATGGACCTCTGTGGAGTTTAG |
| PPARα2 | TTCCAGCTGGCAGAGAGGACGC | CACCCCACAGCCGGAACCACCT |
| PPARβ | GCCAGGGAAAGTGAAGATGAGG | AGCGAGGGCGACGAGGTATG |
| PPARγ | AGAGCGAAGAGCACCTGACC | GCTTGGAGAACACGGGACT |
| ApoA1 | GAGCGTCTGGAGAGTCTGAGG | ACGGAAGCAGCGATGAGC |
| ApoA4 | AGAAGAGCGAGGAACTCAAGGC | GAGGTCGTCGGTGTAGGGTC |
| ApoB100 | TTTTCGCTCTGGGACTCAT | GCTTGCTGGGCTTGTATTT |
| PFK-2 | CAAAGCCGAATCGTCTACTACC | CATCAGGCTTCTGGCATACTCT |
| Cyclin B1 | AAACCCAGTCCCCAAGAAAC | GTCGGTCACGTAGGCAAAGT |
| Cdc2 | ACCCCAGAACCTCCTGATTGAC | AGGCTTCTTGGTGGCGAGTT |
| MYC | GACGGTGGACAGGCGAAAG | GGCGTAGTTGTGCTGATGGATG |
| Cdc20 | AAGGCTTTGGGCTGGTGTC | TCTGTCTTCGTGTCCGTTGAG |
| AURKA | TCACCTCAGGCACCCCAACA | CCTCAGGAAAAGATCCACAGCG |
| Cyclin I | ATAGGCTCTGTTAAAGACCTGGTTGC | GGGCGTGGAATATGTGAATGAAGT |
| β-actin | CAACTGGGATGACATGGAGAAG | TTGGCTTTGGGGTTCAGG |
| Reverse transcription PCR for cloning internal sequence fragments | ||
| DGAT | CTACCCAGGMAACCTCACMC | AAGCGRCCCACGAACCAAGC |
| AURKA | GAGCCTGGARAAYTTTGASATTGG | CATCTGYCAGYTCCATGATGTAHGT |
SCD1, stearoyl-CoA desaturase 1; ApoE, apolipoprotein E; CPT-1α, carnitine O-palmitoyltransferase-1α; FABP1, fatty acid-binding proteins 1; PEPCK, phosphoenolpyruvate carboxykinase; GS, glycogen synthase; LPL, lipoprotein lipase; FAS, fatty acid synthetase; ME, malic enzyme; 6GPD, glucose-6-phosphate dehydrogenase; SREBP1, sterol-regulatory element binding proteins 1; DGAT, diacylglycerol acyl transferase; GPAT, glycerol-3-phosphate acyltransferase; ACO1, acyl-CoA oxidase 1; PPAR, peroxisome proliferator-activated receptor; PFK-2,6-phosphofructo-2-kinase (PFK-2); Cyclin B1, G2/mitotic-specific cyclin-B1; Cdc2, cyclin-dependent kinase 1 (CDK1); MYC, myc proto-oncogene protein; Cdc20, Cell division cycle protein 20; AURKA, aurora kinase A.