Fig. 3.

Role of WBP2 in doxorubicin-induced cell apoptosis in MCF-7 and MCF-7/ADR cells. a Cell apoptosis analysis utilising flow cytometry in control MCF-7 cells and WBP2-overexpressing MCF-7 cells treated with 1.0 μM doxorubicin for 24 h. b Detection of cell apoptosis utilising flow cytometry in MCF-7/ADR cells and RNAi-mediated WBP2 knockdown in MCF-7/ADR cells treated with 1.0 μM doxorubicin for 72 h. c Cell lysates from the control MCF-7, control MCF-7-WBP2, drug-treated MCF-7 and drug-treated MCF-7-WBP2 cells were separated by 8–12% SDS-PAGE, blotted and probed with antibodies against caspase-3, PARP, cleaved PARP, GFP and WBP2. Tubulin served as the internal control. d The expression of above four antibodies (caspase-3, PARP, cleaved PARP and WBP2) were also detected in the control MCF-7/ADR, control MCF-7/ADR-siWBP2, drug-treated MCF-7/ADR and drug-treated MCF-7/ADR-siWBP2 cells by western blotting. Tubulin served as the internal control. EGFP, control MCF-7 cells; EGFP-WBP2, WBP2 stable expression MCF-7 cells; siNC, control MCF-7/ADR cells; siWBP2, RNAi-mediated WBP2 knockdown in MCF-7/ADR cells