Table 5.
Overview of life stage association results for each OTU. Asterisks denote. The OTUs martynovi-complex, H. 683, and H. 685, formed a single GMYC cluster in CAD, here referred to as ‘martynovi-clade’. Key: † indicates larvae without DNA data, * indicates conflicts in COI and CAD gene tree topologies.
| OTU | Samples | Species | Larvae | Larval type | CAD units (PTP/GMYC) | COI units (PTP/GMYC) | Association with CAD (PTP/GMYC) | Association with COI (PTP/GMYC) | Reciprocal monophyly (both genes) | Number of criteria fulfilled |
|---|---|---|---|---|---|---|---|---|---|---|
| H. acharai | 4 | H. acharai | yes | japonica type | 1/1 | 1/1 | yes/yes | yes/yes | yes | 5 |
| H. anomala | 6 | H. anomala | yes | tibetana type | 1/1 | 4/3 | yes/yes | no/yes | yes | 4 |
| H. auricularis | 2 | H. auricularis | no | unknown | 1/1 | 2/1 | – | – | not applicable | – |
| H. biansata | 2 | H. biansata | no | unknown | no data | 1/1 | – | – | not applicable | – |
| H. diehli | 1 | H. diehli | no | unknown | 1/1 | 1/1 | – | – | not applicable | – |
| H. digitata | 34 | H. digitata | yes | tibetana type | 2/1 | 2/2 | yes/yes | no/no | yes | 3 |
| H. eos | 2 | H. eos | no | unknown | no data | 1/1 | – | – | not applicable | – |
| excisa-complex | 31 | H. excisa, H. placida, H. maitreya | yes | tibetana type | 1/1 | 5/2 | no/no | no/no | no | 0 |
| H. gigantea | 2 | H. gigantea | no | gigantea type | no data | 1/1 | – | – | not applicable | – |
| H. gregoryi | 77 | H. gregoryi | yes | tibetana type | 1/1 | 1/1 | yes/yes | yes/yes | yes | 5 |
| H. gyamo | 1 | H. gyamo | no | unknown | 1/1 | 1/1 | – | – | not applicable | – |
| H. horai | 5 | H. horai | no | unknown | 1/1 | 1/1 | – | – | yes | – |
| japonica-complex* | 8 | H. japonica, H. sp. n. 1529 | yes† | japonica type | 2/1 | 4/3 | – | – | no | – |
| H. kuldschensis | 2 | H. kuldschensis | no | unknown | 1/1 | 1/1 | – | – | yes | – |
| H. lanceolata | 4 | H. lanceolata | no | unknown | 1/1 | 1/1 | – | – | yes | – |
| H. lepcha | 5 | H. lepcha | no | unknown | 1/1 | 2/2 | – | – | yes | – |
| H. lua | 5 | H. lua | no | unknown | no data | 2/1 | – | – | not applicable | – |
| martynovi-complex | 30 | H. martynovi, H. epikur | yes | tibetana type | martynovi–clade | 1/1 | no/no | no/no | no | 0 |
| H. navasi | 4 | H. navasi | no | unknown | 1/1 | 4/4 | – | – | not applicable | – |
| H. phryganea | 4 | H. phryganea | yes | phryganea type | 1/1 | 2/2 | yes/yes | yes/yes | yes | 5 |
| platon-complex | 27 | H. platon, H.. sp (F), H.. sp (L) | yes | tibetana type | 1/1 | 4/3 | no/no | no/no | yes | 1 |
| H. sp. 1196 (L) | 17 | NA | yes | tibetana type | 1/1 | 2/1 | – | – | yes | – |
| H. sp. 1254 (L) | 4 | NA | yes | tibetana type | 1/1 | no data | – | – | not applicable | – |
| H. sp. 1338 (L) | 1 | NA | yes | gigantea type | 1/1 | 1/1 | – | – | not applicable | – |
| H. sylvicola | 33 | H. sylvicola | yes | gigantea type | 1/1 | 6/3 | yes/yes | no/yes | yes | 5 |
| H. tibetana | 58 | H. tibetana | yes | tibetana type | 1/1 | 2/2 | yes/yes | yes/yes | yes | 5 |
| H. todma | 4 | H. todma | no | unknown | 1/1 | no data | – | – | not applicable | – |
| triloba-complex* | 67 | H. triloba, H. hageni, H. macilipennis, H. malenanda, H. efiel, H. yatrawalla | yes | gigantea type | 2/2 | 5/3 | no/no | no/no | no | 0 |
| H. yongma | 1 | H. yongma | no | unknown | 1/1 | 1/1 | – | – | not applicable | – |
| H. 677 | 41 | H. 677 | yes | tibetana type | 1/1 | 1/1 | yes/yes | yes/yes | yes | 5 |
| H. 683 | 8 | H. 683 | no | tibetana type | martynovi–clade | 1/1 | – | – | yes | – |
| H. 685 | 34 | H. 685 | yes | tibetana type | martynovi–clade | 1/1 | no/no | yes/yes | yes | 3 |