Figure 1.

Heat map showing skin biopsy discovery cohort global microRNA expression levels. Each row is representative of one microRNA and each column represents one sample. Unsupervised hierarchical clustering analysis was performed using the expression levels from n = 186 detectable microRNAs in the total discovery cohort (n = 9). The heatmap shows the top 50 microRNAs that were expressed in all 9 samples. The vertical dendrogram indicates three distinct microRNA clusters, based on the expression values in all samples. The scale of the dendrogram shows the distance, representing similarity between clusters. The horizontal dendrogram shows the clustering of samples into two clusters. Normalized log₂ values were used for this analysis. The average linkage method was used for the unsupervised hierarchical clustering of the normalized expression results. Fold change range is from −5 to 10. Red boxes highlight microRNAs of interest. Over expressed = green cells, intermediate = light purple cells, under-expressed = dark purple cells. Post allo-HSCT samples are also labeled with their histopathological skin aGvHD stage (1–3) and overall clinical aGvHD grade (II–IV). Abbreviations: N, normal healthy control; allo-HSCT, allogeneic hematopoietic stem cell transplantation; aGVHD, acute graft-versus-host disease.