Figure 3.

The inhibition of RIPK1 by Nec-1 decreases surgery-induced necroptosis in D-Gal-induced aged mice. (A) Representative images of propidium iodide (PI) staining (red) in the dentate gyrus. Nuclei were stained by 4′,6-diamidino-2-phenylindole (DAPI). The arrows indicated some typical cells stained by PI. Bar = 50 μm. (B) The PI-positive cells were counted in the DG. (C,D) Western blot analysis of RIP1 (C) and NF-κB (D) in the hippocampus. The upper panel showed the protein bands of RIP1 and NF-κB, and GAPDH was reference control. The lower panel showed that relative integrated density value of RIP1 or NF-KB to GAPDH. Data were expressed as the mean ± SEM. ^#P < 0.05, compared with control group; *P < 0.05, in comparison between Sur+DMSO and Sur+Nec1 groups by two way ANOVA followed by Tukey’s multiple comparison test (n = 4 per time point per group).