Table 1.
Primers and probes used for detection of F. tularensis and Y. pestis.
| Agent | Gene target | Primer and probe | Sequence (5′ to 3′) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
| F. tularensis | ISFtu2 (chromosome) | ISFtu2F | TTGGTAGATCAGTTGGTAGGATAACC | 97 | Versage et al., 2003 |
| ISFtu2R | TGAGTTTTATCCTCTGACAACAATATTTC | ||||
| Probe | FAM-AAAATCCATGCTATGACTGATGCTTTAGGTAATCCA-TAMRA | ||||
| fopA (chromosome) | fopA-F | AACAATGGCACCTAGTAATATTTCTGG | 87 | Bushon et al., 2010 | |
| fopA-R | CCACCAAAGAACCATGTTAAACC | ||||
| Probe | FAM-TGGCAGAGCGGGTACTAACATGATTGGT-5-TAMRA | ||||
| Y. pestis | yihN (chromosome) | Chrom F | CGCTTTACCTTCACCAAACTGAAC | 128 | Stewart et al., 2008 |
| Chrom R | GGTTGCTGGGAACCAAAGAAGA | ||||
| Probe | Texas Red-TAAGTACATCAATCACACCGCGACCCGCTT-BHQ-2 | ||||
| caf1 (plasmid) | pMT1 F | CCGTTATCGCCATTGCATTATTTGG | 194 | ||
| pMT1 R | GCCAAGAGTAAGCGTACCAACAAG | ||||
| Probe | FAM-AAGCACCACTGCAACGGCAACTCTT-BHQ-1 | ||||
| pla (plasmid) | pPCP1 F | ATTGGACTTGCAGGCCAGTATC | 144 | ||
| pPCP1 R | ATAACGTGAGCCGGATGTCTTC | ||||
| Probe | FAM-AAATTCAGCGACTGGGTTCGGGCACA-BHQ-1 |