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. 2018 May 15;38(3):BSR20170939. doi: 10.1042/BSR20170939

Figure 4. UA enhances vascular endothelial cell apoptosis in rats with MCAO.

Figure 4

(A) The apoptotic cell in each group were visualized under an episcopic-fluorescence microscopy. (B) Statistical analysis of the apoptotic cell rate in each group. Aortic endothelial cells were isolated from rats as previously described [17]. **P<0.01 compared with Sham group. #P<0.5 and ##P<0.01 compared with MCAO + saline group. An in situ apoptotic cell death detection kit TMR red (Roche Applied Science, Indianapolis, IN) based on a TUNEL assay was used to detect apoptotic cells. The apoptotic rate (%) is expressed as the number of apoptotic cells over the total number of cells. **P<0.01 compared with Sham group. #P<0.5 and ##P<0.01 compared with MCAO + saline group. Red indicates the apoptotic cells and blue indicates nucleus.