Table 2.
Shared small ncRNAs identified in top 100 most abundant sequences under normoxia in all four species.
| Small ncRNA sequence | Annotation simplified | Known response in literature | Top 100 most abundant in annual killifish embryos |
|---|---|---|---|
| TGAGGTAGTAGGTTGTATAGTTT |
Let-7 | Stress and hypoxia and preconditioning and stroke- responsive | |
| TGAGGTAGTAGGTTGTATAGTT∗ |
12 dpd embryos | ||
| TGAGGTAGTAGGTTGTATAGTT∗ | |||
| TTCAAGTAATCCAGGATAGGC∗ |
mir-26 | Stress and hypoxia and stroke-responsive | |
| TTCAAGTAATCCAGGATAGGCT∗ |
|||
| TGTAAACATCCCCGACTGGA∗ |
mir-30 | Stress and hypoxia and preconditioning and stroke-responsive | |
| TGTAAACATCCCCGACTGGAAGCT | |||
| TATTGCACTCGTCCCGGCCT∗ |
mir-92 | Stress and hypoxia and preconditioning and stroke-responsive | |
| TATTGCACTCGTCCCGGCCTC∗ |
|||
| TATTGCACTTGTCCCGGCCTGT∗ | 4 dpd and 12 dpd embryos | ||
| AACCCGTAGATCCGATCTTGTG∗ | mir-99 | Stress and preconditioning and stroke-responsive | |
| AACCCGTAGATCCGAACTTG∗ |
mir-100 | Stress and stroke- responsive | |
| AACCCGTAGATCCGAACTTGT∗ |
|||
| AACCCGTAGATCCGAACTTGTG∗ | 12 dpd embryos | ||
| TCCCTGAGACCCTAACTTGTG |
mir-125 | Stress and hypoxia and stroke-responsive | |
| TCCCTGAGACCCTAACTTGTGA∗ | |||
| CATTATTACTTTTGGTACGCG∗ | mir-126 | Hypoxia and stroke responsive | |
| TCACAGTGAACCGGTCTCTTT∗ | mir-128 | Stroke-responsive | 4 dpd and 12 dpd embryos |
| TGAGATGAAGCACTGTAGCT∗ | mir-143 | Stress and stroke-responsive | |
| AACATTCAACGCTGTCGGTGA∗ |
mir-181 | Stress and hypoxia and preconditionin g and stroke-responsive | 4 dpd and 12 dpd embryos |
| AACATTCAACGCTGTCGGTGAG∗ |
|||
| AACATTCAACGCTGTCGGTGAGT∗ |
|||
| AACATTCATTGCTGTCGGTGGG∗ | |||
| TTCCCTTTGTCATCCTATGCCT∗ | mir-204 | Hypoxia and preconditioning and stroke responsive | 4 dpd and 12 dpd embryos |
| AGCTACATCTGGCTACTGGGTCTC∗ | mir-222 | Stress and stroke-responsive | 4 dpd and 12 dpd embryos |
| TCTTTGGTTATCTAGCTGTAT∗ |
Unknown | ||
| TCTTTGGTTATCTAGCTGTATG∗ |
4 dpd and 12 dpd embryos | ||
| TCTTTGGTTATCTAGCTGTATGA∗ | |||
| TGAGGTAGTAGGTTGTATAGT | Unknown | ||
Annotation information, stress response documented in literature, and presence in most abundant small ncRNAs in anoxia tolerant A. limnaeus embryos are included for each sequence (4 dpd, Wourms’ Stage 36 = metabolically active, high anoxia-tolerance; 12 dpd, Wourms’ Stage 40 = metabolically active, intermediate anoxia-tolerance). Asterisk indicates presence of small ncRNA in top 100 most abundant sequences under anoxia as well.