Table 4.
Relative mRNAa expression levels of gyrA, gyrB, mexA, and mexX genes after exposure of PAO1 and its dnpA insertion mutant to different antibiotics.
| Antibiotic |
gyrA |
gyrB |
mexA |
mexX |
||||
|---|---|---|---|---|---|---|---|---|
| PAO1 | dnpA::Tn | PAO1 | dnpA::Tn | PAO1 | dnpA::Tn | PAO1 | dnpA::Tn | |
| Ciprofloxacin | 4.2b | 2.8 | 1.8 | 1.4 | 2.8 | 2.0 | 3.7 | 2.8 |
| (2.5 to 7.1) | (2.0 to 4.0) | (-1.3 to 3.9) | (0.7 to 2.8) | (1.9 to 4.1) | (1.3 to 3.1) | (3.4 to 4.1) | (1.9 to 4.1) | |
| Levofloxacin | 6.0 | 4.0 | 2.2 | 1.9 | 2.3 | 2.3 | 5.4 | 1.6 |
| (4.2 to 8.5) | (3.9 to 4.2) | (1.4 to 3.4) | (1.5 to 2.3) | (1.2 to 4.4) | (1.8 to 2.9) | (4.0 to 7.3) | (1.5 to 1.7) | |
| Meropenem | 1.1 | -1.5 | -1.1 | -1.9 | -1.5 | 1.7 | -1.02 | -2.2 |
| (-1.6 to 2.2) | (-5.0 to 2.2) | (-1.6 to 1.4) | (-5.6 to 1.6) | (-2.3 to 1.1) | (1.4 to 2.0) | (-1.2 to 1.2) | (-4.4 to -1.9) | |
| Amikacin | 1.8 | 1.6 | 1.1 | -1.1 | 1.6 | 1.8 | 1.5 | -3.2 |
| (1.2 to 2.6) | (-1.0 to 2.8) | (-1.7 to 1.9) | (-1.8 to 1.6) | (-1.1 to 3.0) | (1.2 to 2.7) | (1.2 to 1.9) | (-5.4 to -1.9) | |
aRNA was harvested from the surviving bacteria after 5 h of incubation with 50x MIC antibiotic in broth. bcalculated as 2-ΔΔCT, with ΔΔCT = (ΔCT [antibiotic-exposed]-ΔCT [no antibiotic]), and ΔCT = CT [gene of interest] – CT [rspL]. Data are means (with ranges between brackets) of minimum 3 experiments performed in triplicates.