Table 2.

Comparison between the studied groups regarding viability, necrosis, and apoptosis in neurospheres by flowcytometry.

Group/Parameter	Group 1 (control)	Group 2 (TCE -treated group) (TCE: 0.25 μM)	P-value
Viable cells	95.47 ± 0.45	57.17 ± 1.26	0.0005∗∗
Early apoptosis	0.36 ± 0.23	1.60 ± 1	0.2
Late apoptosis	1.5 ± 1.57	19.06 ± 1.29	0.001∗∗
Necrosis	2.67 ± 1.29	22.17 ± 1.04	0.00001∗∗

N.B. TCE, trichloroethylene. All values were expressed as Mean ± SD.

Double negative cells for Annexin V and Propidium iodide (PI) = viable cells, no plasma membrane rupture, Annexin V +, but negative for PI = early apoptotic cells, Double positive cells for both Annexin V and PI = late apoptosis, Positive for PI, but negative for annexin V = necrotic cells (plasma membrane rupture).

P-value: ^∗∗highly significant < 0.001.