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Kinetic profiles of the increase of unconjugated steroids in MCF-7 cells in the presence of RES. Michaelis–Menten plots for the formation of (A) AD, (B) T, and (C) E2 were calculated following the incubation of MCF-7 cells with 0–100 nM DHEA or E1 as hormone precursors in the absence or presence of 0–100 μM RES for 48 h. All data represent the means ± SD of three independent biological replicates.
