Table 1.
Kinetic parameters of RES metabolism by MDA-MB-231 and MCF-7 cells.
| Cell line | Metabolite | Model | Parameters |
||||
|---|---|---|---|---|---|---|---|
| Km (μM) | Vmax (pmol/106 cells/h) | Slope (n) | R2 | Ki (μM) | |||
| MDA-MB-231 | RES-3S | Michaelis–Menten | 9.83 ± 3.18 | 3.25 ± 0.44 | n.a. | 0.9395 | n.a. |
| Substrate inhibition | 14.4 ± 5.6 | 4.31 ± 0.97 | n.a. | 0.9873 | 76.3 ± 35.2 | ||
| RES-3G | Michaelis–Menten | 5.55 ± 3.43 | 32.8 ± 8.6 | n.a. | 0.9522 | n.a. | |
| Substrate inhibition | 8.61 ± 0.85 | 43.4 ± 2.2 | n.a. | 0.9992 | 54.0 ± 5.3 | ||
| MCF-7 | RES-3S | Michaelis–Menten | 7.30 ± 0.44 | 468.4 ± 9.9 | n.a. | 0.9991 | n.a. |
| Substrate inhibition | 8.19 ± 0.56 | 501.4 ± 17.1 | n.a. | 0.9889 | 39.4 ± 14.3 | ||
| RES-3G | Hill | 58.5 ± 6.2 | 422.9 ± 38.9 | 1.86 ± 0.18 | 0.9988 | n.a. | |
Kinetic parameters were calculated using GraphPad Prism 6.0 software following the incubation of the cells with increasing concentrations of RES (0–100 μM) for 48 h. All data represent the means ± SD of three independent biological replicates. n.a., not applicable.