FIGURE 1.

Exogenous H₂O₂ did not inhibit the growth of P. putida MnB1, but was bio-degraded quickly by P. putida MnB1. (A) Cells were incubated with 0, 40, 200, and 1000 μM H₂O₂, respectively. Growth of P. putida MnB1 was detected by measuring the absorbance at 600 nm (n = 3). (B) The concentration of H₂O₂ in culture with P. putida MnB1was assayed by the xylenol orange reaction (n = 3). (C) H₂O₂ concentration (initial concentration of 1000 μM) in LEP medium was assayed in the presence or absence of alive P. putida MnB1 or inactivated microbes, respectively. The supernatant of the cell culture was reacted with xylenol orange. Data were expressed as mean ± SEM. Significance, ^∗∗P < 0.01 and ^∗∗∗P < 0.001 vs. control group (0 μM H₂O₂).