FIGURE 6.

The sod and bifA mRNA levels in biofilm cells as well as during biofilm development with exogenous H₂O₂. sod (A) and bifA (C) mRNA levels were detected by qRT-PCR analysis in colonized cells incubated with 200 μM H₂O₂ for 2 h after 3 h, 6, 12, and 24 h cultivation for adhesion, respectively. Relative gene expression levels of sod and bifA were normalized to 16s rRNA (n = 4), respectively. The cellular mRNA levels of sod (B) and bifA (D) during different stages of biofilm formation. Data were expressed as mean ± SEM. Significance, ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 vs. control group (0 μM H₂O₂). ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. 3 h-biofilm formation group.