FIGURE 9.

Effect of GTP on biofilm formation by P. putida MnB1. Microbes were incubated with Mn²⁺ at 40, 200, and 1000 μM for 6 h (A) and 12 h (B) in the presence or absence of 250 μM GTP (n = 6), respectively. Microbes were incubated with H₂O₂ at 40, 200, and 1000 μM for 6 h (C) and 12 h (D) in the presence or absence of 250 μM GTP (n = 6), respectively. Biofilm formation of P. putida MnB1 was quantified by CV staining with absorbance at 590 nm. Data were expressed as mean ± SEM. Significance, ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 vs. control group (0 μM GTP).