Figure 3.

Neuroinvasiveness of JEV WT and mutants. C57BL/6 mice were intraperitoneally (ip) inoculated with the indicated JEVs at 10⁶ PFU (A) or 10⁵ PFU (B) per mouse and simultaneously injected intracerebrally (ic) with 30 μl PBS (ip plus ic route). The survival of mice was observed daily. Prism software with the log rank test was used to compare survival curves with WT. ^*P < 0.05. (C) RT-qPCR of relative JEV RNA levels in brain, liver, and lymph node tissues of mice inoculated with WT or E-D67N mutant (10⁶ PFU/mouse) (n = 3). (D) 5-week-old Stat-1 knockout mice were subcutaneously infected with JEV E mutants (0.1 PFU/mouse). The number of mice (n) in each experimental group is shown in the legend to each panel. Prism software with the log rank test was used to compare survival curves with WT. ^**P < 0.01.