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. 2018 Jul 10;8:239. doi: 10.3389/fcimb.2018.00239

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Copyright © 2018 Liang, Chou and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Vaccine potential of JEV E-glycosylation mutants. Four-week-old C57BL/6 mice (n = 4 per group) were subcutaneously injected with JEV E-glycosylation mutants at 10¹, 10², or 10³ PFU per mouse. Two weeks after immunization, mice were challenged with 2 × 10⁵ PFU of the JEV WT RP-9 strain by the ip plus ic route. Mouse survival was observed daily. Prism software with the log rank test was used to compare survival curves. ^*P < 0.05.