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Plaque-forming ability of JEV WT and mutants in cells with or without DC-SIGN. BHK-21 cells overexpressing eGFP or DC-SIGN were infected with serially diluted JEV WT or mutants, then overlaid with agarose-containing medium for plaque formation. After incubation for 4 days, cells were fixed with formalin and stained with crystal violet. The number of plaques formed were counted; folds of plaque formation enhanced by DC-SIGN as compared with eGFP control are shown.
