FIG 8.

Telomeric ura4⁺ is silenced. (A) Growth of cells containing the 48-bp prototelomere before and after 20 h of I-SceI induction was assessed by spotting 5-fold serial dilutions of cells onto rich-medium plates (YES [81]) with and without hygromycin (hyg), or synthetic EMMG medium with (+ura) or without (−ura) uracil. (B) The presence of the ura4⁺ gene in untreated 48-bp prototelomere cells prior to induction (−) or in three independent Ura^– hygromycin-sensitive colonies derived from ahTET-treated cells (+) was tested by PCR. Primers are indicated by arrows.