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. 2018 Jul 16;38(15):e00393-17. doi: 10.1128/MCB.00393-17

TABLE 1.

Strains used in this study

Strain Genotype Notes
YSLS793 h− leu1-32::pDUAL-TETp-I-PpoI rDNAI-PpoImt lys1::I-PpoICS-hph+ Reference 34
YSLS792 h− leu1-32::pDUAL-TETp-I-PpoI rDNAI-PpoImt lys1::HOCS-hph+ Reference 34
YJRE49 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR
YJRE107 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR leu1-32::pFA-LEU2-TETp-I-SceI I-SceI enzyme + no cleavage site
YJRE112 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR I-SceI enzyme + I-SceI site at 48-bp prototelomere
YJRE116 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 0-nt TeloRpt-I-SceI-hygR I-SceI enzyme + I-SceI site at 0-bp prototelomere
YJRE140 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR leu1-32::pDUAL-TETp gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR No enzyme + I-SceI site at 48-bp prototelomere
YJRE151 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR leu1-32::pDUAL-TETp gal1-3′::ura4+ 0-nt TeloRpt-I-SceI-hygR No enzyme + I-SceI site at 0-bp prototelomere
YJRE179 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR lys1::I-SceI hygR leu1-32::pFA-LEU2-TETp-I-SceI I-SceI enzyme + I-SceI site at lys1+
YJRE180 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR lys1::I-SceI hygR leu1-32::pDUAL-TETp No enzyme + I-SceI site at lys1+
YJRE181 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR leu1-32::pFA-LEU2-TETp-I-SceI SPBPB2B2.07c repaired 0-bp survivor derived from YJRE116
YJRE182 h− ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48+++-nt TeloRpt 48-bp form derived from YJRE112
YJRE210 h+ ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR I-SceI enzyme + I-SceI site at 48-bp prototelomere
YJRE212 h+ ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 0-nt TeloRpt-I-SceI-hygR I-SceI enzyme + I-SceI site at 0-bp prototelomere
JA007-1 h+ clr4::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR clr4Δ I-SceI enzyme + I-SceI site at 48-bp prototelomere
JA011-1 h+ TER1::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR TER1Δ I-SceI enzyme + I-SceI site at 48-bp prototelomere
JA018-2 chk1-13myc::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR Chk1-myc in I-SceI enzyme + I-SceI site at 48-bp prototelomere
JA046-1 chk1-13myc::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 0-nt TeloRpt-I-SceI-hygR Chk1-myc in I-SceI enzyme + I-SceI site at 0-bp prototelomere
JA057-1 h+ ade6::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR ade6Δ I-SceI enzyme + I-SceI site at 48-bp prototelomere
JA058-1 chk1-13myc::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 hhf1::arg3+ hhf2::his3+ hhf3::natR lys1::I-SceI hygR leu1-32::pFA-LEU2-TETp-I-SceI Chk1-myc in I-SceI enzyme + I-SceI site at lys1+
JA064-1 h+ ade6::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR ade6+ (at kb 20 downstream I-SceI site) ade6+ at kb 20 to I-SceI site (48-bp prototelomere + I-SceI enzyme)
JA066-1 h+ ade6::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR ade6+ (at kb 88 downstream I-SceI site) ade6+ at kb 88 to I-SceI site (48-bp prototelomere + I-SceI enzyme)
JA067-1 h+ ade6::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR ade6+ (at kb 4 downstream I-SceI site) ade6+ at kb 4 to I-SceI site (48-bp prototelomere + I-SceI enzyme)
JA068-1 h+ ade6::kanMX6 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 leu1-32::pFA-LEU2-TETp-I-SceI gal1-3′::ura4+ 48-nt TeloRpt-I-SceI-hygR ade6+ (at kb 16 downstream I-SceI site) ade6+ at kb 16 to I-SceI site (48-bp prototelomere + I-SceI enzyme)