FIG 9.

CD133-EVs are taken up by recipient cells and promote cell migration. EVs were harvested from U87MG cells expressing Flag, CD133-WT, or CD133-K848R. (A) Model of U87MG cell incubation with isolated EVs. (B) The indicated EVs were added to the U87MG cell culture medium. After incubation with EVs for 5 h, the cells were washed with PBS, and the expression of CD133 in cell lysates was detected by Western blotting. The expression of β-actin served as a loading control. (C) Representative images from a Transwell assay of cells treated with equal volumes of CD133-WT-, CD133-K848R-, and Flag-EVs. (D) Cell migration numbers were calculated and are presented as means and SD (n = 6). Statistical analysis was performed using the Student t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). EVs, extracellular vesicles.