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. 2018 Jul 10;9:738. doi: 10.3389/fphar.2018.00738

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Copyright © 2018 Keenan, Langenbach, Jativa, Harris, Li, Chen, Xia, Gao, Schuliga, Jaffar, Prodanovic, Tu, Berhan, Lee, Westall and Stewart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Effect of PF670462 on EMT associated gene induction in A549 alveolar epithelial cells 4 and 24 h after TGF-β (100 pM) stimulation (n = 4). (B) Immunofluorescence of membrane E-cadherin expression in A549 alveolar epithelial cells. Cells were treated with TGF-β (100 pM) for 48 h with PF670462 added 30 min prior to TGF-β. Nuclei were stained with DAPI. Quantification of n = 4 experiments is shown in (C) with >50 fields analyzed per well. Data are presented as mean ± SEM from 4 independent experiments on A549 epithelial cells. ^∗P < 0.05, ^∗∗P < 0.01 from two-way ANOVA with Bonferroni post hoc test or one-way ANOVA with Dunnet’s post hoc test. ^∧P < 0.05 from one-sample t-test compared to 1.0.