Skip to main content
. 2018 Jul 10;9:738. doi: 10.3389/fphar.2018.00738

FIGURE 5.

FIGURE 5

The EMT gene expression at baseline and in the presence of TGF-β (100 pM, 24 h incubation) was measured in the presence of vehicle (Veh, 0.1% DMSO) or over a range of concentrations of PF670462 (0.1 – 10 μM), Pirfenidone (1 –100 μM)) or nintedanib (10 –1000 nM) in. Data are presented as mean and SEM of n = 4 independent experiments and show the TGF-β induced fold increase in the expression of genes that change during EMT or in response to TGF-β in A549 cells, including N-cadherin (N-Cad), Vimentin (Vim), E-Cadherin (E-Cad), α-smooth muscle actin (a-SMA) plasminogen-activator inhibitor-1 (PAI-1). Data were analyzed by two-way ANOVA with repeated measures, followed by comparisons at individual concentrations using Bonferroni’s correction for multiple comparisons. ∗P < 0.05.