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. 2018 Jul 10;9:738. doi: 10.3389/fphar.2018.00738

FIGURE 6.

FIGURE 6

The dual CK1δ/ε inhibitor PF670462 prevents and attenuates bleomycin-induced pulmonary fibrogenesis in mice. Male C57Bl/6 mice received an intranasal dose of bleomycin or saline on day 0. PF670462 (30 mg/kg/day, i.p.). (A,B) p38MAPK phosphorylation and fibrogenic gene expression in lungs from mice treated with PF670462 from day – 1 to 3 as measured on day 3 (n = 5 per group). Western blots of 2 representative mice are shown and data from all mice quantitated and tested. (C) Hydroxyproline content and bronchoalveolar lavage (BAL) cell number in lungs from female mice treated with PF670462 from day 3–13, as measured on day 14 (n = 6 sal, n = 12 bleo groups). (D) Hydroxyproline content and wet weight of lungs and BAL cell number and protein content from female mice treated with PF670462 from day 7–21 as measured on day 21 (n = 7 sal, n = 9–10 bleo groups). Data are presented as mean ± SEM. ∗P < 0.05, ∗P < 0.01, ∗∗∗P < 0.001 from one-way ANOVA with Dunnet’s post hoc test, or two-way ANOVA with Bonferroni post hoc test.