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. 2018 Jul 10;9:990. doi: 10.3389/fpls.2018.00990

FIGURE 6.

FIGURE 6

Medicago truncatula MTP2 mutation impairs nitrogen fixation. (A) Representative WT, mtp2-1, and mtp2-2 plants. Bars: 1 cm. (B) Representative nodules of WT, mtp2-1, and mtp2-2 plants. Bars: 500 μm. (C) Dry weight of shoots (black) and roots (white). Data are the mean ± SD of, at least, 10 plants. Comparisons have been made among the shoots and among the roots. (D) Nitrogenase activity of 28-dpi nodules. Acetylene reduction was measured in duplicate from three sets of four pooled plants. Data are the mean ± SD. (E) Zn content in shoots, roots and nodules of WT (black), mtp2-1 (white), and mtp2-2 (gray). Data are the mean ± SD of three sets of at least ten pooled plants. Values with different letters are significantly different (Tukey’s HSD, p < 0.05).