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. 2018 Jun 1;8(6):964–980.

Figure 2.

Figure 2

Altered GOLM1 expression in cervical cancer cell lines modifies sensitivity to methotrexate. A. MTS analysis of SiHa and Ca-Ski cells transfected with shGOLM1, or negative control (shCon), pLV-GOLM1, negative control (pLV-Vector). B, C. Representative images and statistical analysis of colony formation among SiHa and Ca-Ski cells transfected with shGOLM1 or pLV-GOLM1. D, E. MTS assay and the corresponding IC50 of methotrexate measured in SiHa and Ca-Ski cells transfected with shGOLM1 or pLV-GOLM1. F. Quantification of Annexin V/PI-positive apoptotic cells in SiHa and Ca-Ski cervical cancer cells transfected with shGOLM1 or pLV-GOLM1 after methotrexate treatment (10 nM) via flow cytometric analysis. G. Representative images of the morphological changes of SiHa cells transfected with pLV-GOLM1 or pLV-Vector and Ca-Ski cells with shGOLM1 or shCon. H. EMT-related marker expression in SiHa cells transfected with pLV-hGOLM1 or pLV-Vector were shown by western blotting and qPCR. I. EMT-related marker expression in Ca-Ski cells transfected with shGOLM1 or shCon shown by western blotting and qPCR. **P < 0.01, compared with control.