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. 2018 Jun 1;8(6):964–980.

Figure 4.

Figure 4

GOLM1 sensitizes cervical cancer cells to methotrexate is MMP13-dependent. A-D. qPCR and western blotting assays confirming the overexpression or knock down of MMP13 in both SiHa and Ca-Ski cells. E, F. MTS assay and the methotrexate IC50 measurements in SiHa and Ca-Ski cells that transfected with the MMP13 shRNA or pLV-MMP13. G. The percentage of apoptotic cells in cervical cancer cells transfected with the MMP13 shRNA or pLV-MMP13 using flow cytometric analysis. H, I. Comparison of cell viability and the IC50 of methotrexate in SiHa and Ca-Ski cells with pLV-GLOM1 alone or pLV-GLOM1 combined with shMMP13 transfection. J. Apoptosis assays in SiHa and Ca-Ski cells with pLV-GLOM1 alone or pLV-GLOM1 combined with shMMP13 transfection. **P < 0.01, compared with control, ##P < 0.01, compared with pLV-GOLM1/shCon.