Figure 5.

Effects of SCIN overexpression on SMMC-7721 cells. (A) Exogenous SCIN level in SMMC-7721 cells infected with lentivirus overexpressing flag-tagged SCIN (OE) or empty vector (Ctl) was investigated by western blot. β-actin was used as a loading control. (B) Representative flow cytometric images of lentivirus-infected SMMC-7721 cells determined from cell cycle analysis. Cell growth curves of lentivirus-infected SMMC-7721 cells under normal culture conditions (C) and serum deprivation (D). Values are indicated as mean ± SD, n = 5. Lentivirus-infected SMMC-7721 cells treated with indicated dosages of cisplatin (cis) were subjected to cell apoptotic analysis through sub-G1 analysis (E) and caspase 9 immunoblot (F). Values are indicated as mean ± SD, n = 3.