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. 2018 Jun 1;8(6):1030–1039.

Figure 3.

Figure 3

miR-26a directly targeted ARPP-19 in TPC-1 cells. A. Sequences complementary pairings of miR-26a with ARPP19 wild-type and mutant 3’ UTR were shown. B. mRNA levels of ARPP19 in TPC-1 cells transfection with mimics-NC, mimics of miR-26a or inhibitor-NC, inhibitor of miR-26a were determined by qRT-PCR. C. Protein levels of ARPP19 in TPC-1 cells transfection with mimics-NC, mimics of miR-26a or inhibitor-NC, inhibitor of miR-26a were determined by western blot. Representative images of western blot were shown, bands were quantitated by densitometry and normalized against GAPDH. D. Luciferase activities were determined in TPC-1 cells 48 h after co-transfection with the mimics-NC, mimics of miR-26a, inhibitor-NC or inhibitor of miR-26a and Luciferase reporter vector containing WT or mutants of APRR19 3’ UTR. **, P<0.01 compared with mimics-NC; ##, P<0.01 compared with inhibitor NC.