Figure 1.

MiR-125b is aberrantly expressed in HCCs and is associated with sirtuin family. A. The miR-125b expression level in clinical samples was analyzed by qPCR. B. miR-125b is differentially expressed in HCC patients and HTSeq-Counts data acquired from TCGA database, which is shown with a logarithmic conversion (log2^counts). Unpaired t-test. C. miR-125b gene is related with the overall survival rate of HCC patients. Red line: high expression, blue line: low expression. Data is acquired from TCGA database and analyzed via LinkedOmics bioinformatics [23]. D. Schematic illustrations showing exact position and numbers of miR-125b binding sites of certain sirtuin family members. E. The predicted binding site of miR-125b in the 3’UTR of SIRT2, SIRT3, SIRT5 SIRT6 and SIRT7 mRNA is analyzed via TargetScan bioinformatics [24]. F. Western blots shows the protein level of SIRT1-SIRT7 in cells transfected with miR-125b mimic or control mimic in HepG2 cell lines. G. The expression of SIRT1-SIRT7 in HCC patients. The results, based on TCGA database, were acquired from GEPIA bioinformatics [25].