Figure 6.

MiR-125b induces HCC senescence and apoptosis via inhibiting SIRT6. A. Senescence-associated β-galactosidase staining was used to exhibit senescence-like phenotypes in SIRT6 knockout HepG2 and Sk-hep-1 cell lines. Cells with positive staining were shown in a representative photograph. Positive staining cell counts were presented as the mean ± SD, n=3. Statistical analysis was performed using t-test. B. SA-β-gal staining assay showing the cell senescence rate in miR-125b overexpression cells and miR-125b overexpression HepG2 and Sk-hep-1 cells upon SIRT6 transfection. C. Western blots shows the SIRT6, P16, Bax and Caspase3 protein level in cells of each group as treated above. β-actin was used as loading control.