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. 2018 Jul 10;9:920. doi: 10.3389/fpls.2018.00920

FIGURE 2.

FIGURE 2

Qualitative analysis specific to bar gene and its functionality. (A) Screening of transgenics by CPR assay. After incubation, media (with 10 mg/l PPT) color changed to yellow-orange for transgenic leaf (with bar gene) and red-purple for bar negative WT plant leaf. Here the transgenic plants JBC1.1, JBC1.2, and JBC1.3 represent the JBC1 line, JBC2.1, JBC2.2, and JBC2.3 represent the JBC2 line, and JBC3.1 and JBC3.2 represent the JBC3 transgenic line. (B) The bar positive leaf containing media showing pH range of 3.20–4.20 with significant difference (∗∗P = < 0.01, ∗∗∗P = < 0.001) as compared to WT by one-way ANOVA at P < 0.05 and Tukey’s multiple comparisons tests using Graphpad Prism 6 software. (C) In ammonia assimilation assay leaf disks (5 mm) incubated in 10 mg/l PPT containing incubation media for 8 h in light. (D) Change in color of the solution judged as either positive (pale blue to yellow color) or negative (dark blue color) plants. Here, JBC1.1 transgenic plant represent the JBC1 line, JBC2.2 represent the JBC2 line and JBC3.1 represent the JBC3 transgenic line. (E) Yellow color developed in positive wells of QualiPlate ELISA plate. OD measured at 450 nm and significant difference (∗∗∗P = < 0.001) found between transgenic and WT samples by Tukey’s multiple comparison tests. OD ≥ 0.2 is considered as PAT/bar positive. Here, specific individual plants are the representatives of the three transgenic lines JBC1, JBC2, and JBC3.