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. 2018 Jul 10;9:920. doi: 10.3389/fpls.2018.00920

FIGURE 7.

FIGURE 7

Detached leaf antifungal bioassay of transgenic plants. (A) PDA plate of M. phaseolina pure culture. Black line indicates the peripheral zone from where 5 mm disks of inoculum was taken for fungal bioassay. (B) Schematic diagram of a JRC-321 control jute leaf with three distinct lesion areas (infected, invaded, and responsive area) developed by M. phaseolina infection. (C) A mock JRC-321 jute leaf (WT without M. phaseolina inoculation) bio-assayed with a PDA disk. (D) After 72 h of bioassay, reduction in invaded lesion area (white circled) observed in transgenic leaves compared to non-transgenic WT. No such area formed in mock JRC-321 leaf. Experiments replicated three times. (E) A significant difference (∗∗∗∗P < 0.0001) in relative lesion area percentage between transgenic progenies and WT recorded from the one-way ANOVA at P < 0.05 and Tukey’s multiple comparisons analysis in Graphpad Prism 6 software. Here, three individual plants JBC1.1, JBC2.1, and JBC3.1 are the representatives of the three transgenic lines JBC1, JBC2, and JBC3, respectively.