Table 1.
List of primers used in this investigation (Designed by PrimerQuest® software and synthesized through Integrated DNA Technologies, United States).
| Gene (accession number) | Primer name | Primer sequence (5′ to 3′) | Amplicon size and anneling temperature | Use of PCR amplified product |
|---|---|---|---|---|
| Rice chitinase (X54367) | CHI-473-F | TCGCCTCCATCATATCGCCCTC | 473 bp and 53.0°C | PCR-screening, Probe preparation in Southern |
| CHI-473-R | CGTCATCCAGAACCAGAACGCC | hybridization | ||
| RT-CHI-F | GTTCTGGTTCTGGATGACGC | 139 bp and 59.0°C | RT-PCR, qRT-PCR | |
| RT-CHI-R | GCCGTTGATGATGTTGGTGA | |||
| bar (JQ293091) | BAR-555-F | CGCCGATGGTTTCTACAAAGA | 555 bp and 60.5°C | PCR-screening, Vector construction |
| BAR-555-R | TCAATGACCGCTGTTATGCG | |||
| RT-BAR-F | CTACACCCACCTGCTGAAG | 157 bp and 59.0°C | RT-PCR, qRT-PCR | |
| RT-BAR-R | CCAGTTCCCGTGCTTGAAG | |||
| Jute 26S rRNA (JK743816) | RT-26S-F | GTTCCACACGAGATTTCTGTTC | 145 bp and 59.0°C | RT-PCR, qRT-PCR (as internal control) |
| RT-26S-R | TTTTAGACCCAAGACCGGC |