Table 2.
Segregation analysis of transgene (bar) in advanced T2 generation progeny plants.
| Progeny plants | Plant survivability % in PPT (10 mg/l) | PPT resistant | PPT sensitive | PPT resistant: PPT sensitive | Best fit segregation ratio | Chi square value | P-value |
|---|---|---|---|---|---|---|---|
| JBC1.1.1 | 74.50 | 149 | 51 | 2.92:1 | 3:1 | 0.027 | 0.870 |
| JBC2.1.1 | 76.00 | 152 | 48 | 3.16:1 | 3:1 | 0.107 | 0.744 |
| JBC3.1.1 | 72.50 | 145 | 55 | 2.63:1 | 3:1 | 0.667 | 0.414 |
Plant survivability % = (number of PPT resistant plants/total plants) × 100. The two-tailed P-value has been represented and Chi square value has been represent with 1 degree of freedom. PPT (phosphinothricin) or glufosinate ammonium or glufosinate, is a herbicide. Here, the three transgenic lines of JBC1, JBC2, and JBC3 have been represented by the individual transgenic T2 plants JBC1.1.1, JBC2.1.1, and JBC3.1.1, respectively.