Table 1.
Primer sequences for real-time quantitative PCR
| Gene | Sense (5′–3′) | Antisense (5′–3′) |
|---|---|---|
| Rock1 | GACTGGGGACAGTTTTGAGAC | GGGCATCCAATCCATCCAGC |
| Skp1a | ATGCCTACGATAAAGTTGCAGAG | TCCATTCCCAAATCTTCCAGC |
| Tbl1xr1 | TCTCATTCTGCGTTTACCTTTGG | GACAGAGACTCGATGGGTC |
| Ppp2r5e | GACGGATTTTCTCGGAAGTCC | GAGGTTGGAACGTCTTTCAGC |
| Plcb1 | GCCCCTGGAGATTCTGGAGT | GGGAGACTTGAGGTTCACCTTT |
| Let1 | TGTTTATCCCATCACGGGTGG | CATGGAAGTGTCGCCTGACAG |
| Camk2b | GCACGTCATTGGCGAGGAT | ACGGGTCTCTTCGGACTGG |
| Prickle1 | ACCTGGAGTATGCTGGCAC | CACAGTGGATTTTTCCATCCTGA |
| Ppp2r5a | ATTGAAGAGCCGCTTTTTAAGCA | TGAGGGTTTTCAGCACATTGT |
| Daam1 | AACTTTGCACTTCAGACAATGGA | CTGGTCCTTTTTCTTGCTACAGT |
| Ppp3r2 | AAATGAGGCCAGCTACCAAAC | CCCGATTTGTCCAAGTCCAG |
| Cxxc4 | CTGCCCGCAGAATCATTCCT | CAGACGCCACAGTTGATGAG |
| Smad4 | ACACCAACAAGTAACGATGCC | GCAAAGGTTTCACTTTCCCCA |
| Nfat5 | CAGCGCCCAATAGTTGGCA | TGCTGGTGAAAAATTGACTGGT |
| Actb | CCTTCTTGGGTATGGAAT | TTGGCATAGAGGTCTTTA |