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. 2018 Jul 10;9:986. doi: 10.3389/fpls.2018.00986

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Copyright © 2018 Arnaiz, Talavera-Mateo, Gonzalez-Melendi, Martinez, Diaz and Santamaria.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the expression, location and inhibitory properties of AtKTI4 and AtKTI5 genes by using transient expression assays in N. benthamiana plants. (A) GFP signal after 3 days of agroinfiltration. Asterisks indicate GFP signal of the epidermal cells. (B) Relative expression levels of GFP, KTI4, and KTI5 genes in the N. benthamiana plants after 3 days of infiltration. (C) Inhibitory ability of N. benthamiana protein extracts expressing KTI4 and KTI5 genes against commercial trypsin, chymotrypsin, papain and bovine cathepsin B 3 days post-agroinfiltration. Data are mean ± SE of triplicate measurements of each sample. Asterisk indicates significant differences with the 35S::GFP plants (P < 0.05, One-way ANOVA followed by Dunnett’s test).