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Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) defines genome-wide transcriptional regulation by pSmad1 protein upon growth differentiation factor 15 (GDF15) in human airway epithelial cells. Normal human tracheobronchial epithelial cells in submerged culture were treated with 0.1% BSA-HCl (control) or recombinant human GDF15 (25 ng/ml) for 2 h to perform the phosphorylated-Smad1 (pSmad1) ChIP-seq analysis.
