Fig. 7.

Human Treg proliferation ex vivo from diseased liver is associated with glycolytic and FAS-related gene expression. OX40⁺/OX40⁻ CD45RA^low Tregs and Tconvs were extracted and sorted from the PB and CT of five patients with chronic HCV infection, and gene expression analysis was performed. (A) Sorting strategy. (B) The fold-change was calculated for each gene over the respective control (OX40⁻ PB Tconvs for all Tconv samples and OX40⁻ PB Tregs for all Treg samples), and 216 genes up-regulated more than twofold only in Treg OX40⁺ CT were selected for pathway analysis (https://reactome.org/). The heatmap displays the fold-change over control in the genes accounting for two pathways of interest among those showing statistically significant enrichment (P < 0.05).