Fig. 3.

SEVI alters rVSV-EboGP-mCherry physical characteristics. (A) rVSV-EboGP-mCherry was incubated at 37 °C in artificial semen simulant with or without 35 μg/mL SEVI fibrils, SEVI-Ala fibrils, or PAP248–286 peptide and titered by TCID₅₀ on Vero cells. In the 0 μg/mL + 35 μg/mL SEVI condition, SEVI was added immediately before titration after incubation without SEVI; n = 3, mean ± SEM. (B) rVSV-EboGP-mCherry was incubated at 37 °C in 10% seminal plasma or seminal plasma filtrate, then titered by TCID₅₀ on Vero cells; n = 3, mean ± SEM. (C) rVSV-EboGP-mCherry was diluted in artificial semen simulant with or without 35 µg/mL SEVI fibrils and dried under laminar flow before rehydration and titration by TCID₅₀ on Vero cells; n = 3, mean ± SEM. (D) rVSV-EboGP-mCherry was diluted in 10% seminal plasma or seminal plasma filtrate and dried under laminar flow before rehydration and titration by TCID₅₀ on Vero cells; n = 3, mean ± SEM.