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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Stem Cells. 2017 Jun 27;35(8):1913–1923. doi: 10.1002/stem.2646

Figure 2.

Figure 2

YY1 overexpression inhibits cardiomyogenic differentiation of ES cell-derived CPCs. (A) Schematic diagram of in vitro cardiac differentiation of the NK-YY1 ESC line. NK-YY1 ESC line underwent monolayer differentiation in vitro for 6 days and eGFP+ cells were purified by FACS, replated, and cultured in the presence or absence of doxycycline to induce YY1 overexpression for an additional 4 days until day 10. (B) Bright field (BF) and fluorescence (GFP) microscopy analysis of FACS-purified eGFP+ cells after 4 days of YY1 overexpression. (C) Quantitative PCR analysis of YY1 expression in the presence or absence of doxycycline treatment for 4 days. (D) Quantification of cell number in each well after YY1 overexpression. (E) Quantitative PCR analysis of cardiac transcription factor expression after YY1 overexpression. (F) Quantitative PCR analysis of sarcomeric gene expression in YY1 overexpressed vs control cells. Three independent experiments were performed in triplicate. (***) P<0.001. Scale bar: 50μm.