Skip to main content
. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Stem Cells. 2017 Jun 27;35(8):1913–1923. doi: 10.1002/stem.2646

Figure 3.

Figure 3

Dominant negative YY1 expression promotes maturation of ESC-derived CPCs into differentiated cardiomyocytes. (A) Schematic diagram of in vitro cardiac differentiation of the NK-YY1-DN ESC line. NK-YY1-DN ESC line underwent monolayer differentiation in vitro for 6 days and eGFP+ cells were purified by FACS, replated, and cultured in the presence or absence of doxycycline to induce YY1-DN overexpression for 4 additional days until day 10. (B) Bright field (BF) and fluorescence (GFP) microscopy analysis of FACS-purified eGFP+ cells after 4 days of YY1-DN overexpression. (C) Quantitative PCR analysis of YY1-DN expression in the presence or absence of doxycycline. (D) Quantification of cell number in each well after YY1-DN overexpression. (E) Quantitative PCR analysis of cardiac transcription factor expression after YY1 overexpression. (F) Quantitative PCR analysis of sarcomeric gene expression in YY1-DN overexpressed vs control cells. Three independent experiments were performed in triplicate. (***) P<0.001, (**) P<0.05. Scale bar: 50μm.