Table 2.
Two-way ANOVA statistical analysis of results for Smart Matrix.
| Passage number/attachment incubation time | ||||||
|---|---|---|---|---|---|---|
| Source of variation | SS | df | MS | F | P-value | F crit |
| Passage number | 627.5196297 | 1 | 627.5196 | 0.814573 | 0.377513 | 4.351244 |
| Attachment incubation time | 7110.063006 | 1 | 7110.063 | 9.229461 | 0.006495 | 4.351244 |
| Interaction | 47.89944573 | 1 | 47.89945 | 0.062178 | 0.80563 | 4.351244 |
| Within | 15,407.31953 | 20 | 770.366 | – | – | – |
| Total | 23,192.80162 | 23 | – | – | – | – |
| Passage number/scaffold disc to well plate surface area ratio | ||||||
| Source of variation | SS | df | MS | F | P-value | F crit |
| Passage number | 627.5196297 | 1 | 627.5196 | 0.923124 | 0.348129 | 4.351244 |
| Scaffold disc/well plate surface area | 8676.122015 | 1 | 8676.122 | 12.76316 | 0.001906 | 4.351244 |
| Interaction | 293.5879223 | 1 | 293.5879 | 0.431888 | 0.518556 | 4.351244 |
| Within | 13,595.57205 | 20 | 679.7786 | – | – | – |
| Total | 23,192.80162 | 23 | – | – | – | – |
| Passage number/cell seeding density | ||||||
| Source of variation | SS | df | MS | F | P-value | F crit |
| Passage number | 627.5196297 | 1 | 627.5196 | 0.526167 | 0.477548 | 4.413873 |
| Cell seeding density | 1016.717587 | 2 | 508.3588 | 0.426252 | 0.659373 | 3.554557 |
| Interaction | 81.33679455 | 2 | 40.6684 | 0.0341 | 0.966537 | 3.554557 |
| Within | 21,467.2276 | 18 | 1192.624 | – | – | – |
| Total | 23,192.80162 | 23 | – | – | – | – |
| Scaffold disc to well plate surface area ratio/attachment incubation time | ||||||
| Source of variation | SS | df | MS | F | P-value | F crit |
| Scaffold disc/well plate surface area | 8676.122015 | 1 | 8676.122 | 39.54241 | 3.87E-06 | 4.351244 |
| Attachment incubation time | 7110.063006 | 1 | 7110.063 | 32.40492 | 1.43E-05 | 4.351244 |
| Interaction | 3018.355262 | 1 | 3018.355 | 13.7565 | 0.001388 | 4.351244 |
| Within | 4388.261333 | 20 | 219.4131 | – | – | – |
| Total | 23,192.80162 | 23 | – | – | – | – |
| Attachment incubation time/cell seeding density | ||||||
| Source of variation | SS | df | MS | F | P-value | F crit |
| Attachment incubation time | 7110.063006 | 1 | 7110.063 | 9.077012 | 0.007474 | 4.413873 |
| Cell seeding density | 1016.717587 | 2 | 508.3588 | 0.648993 | 0.534373 | 3.554557 |
| Interaction | 966.542881 | 2 | 483.2714 | 0.616965 | 0.550605 | 3.554557 |
| Within | 14,099.47814 | 18 | 783.3043 | – | – | – |
| Total | 23,192.80162 | 23 | – | – | – | – |
| Scaffold disc to well plate surface area ratio/cell seeding density | ||||||
| Source of variation | SS | df | MS | F | P-value | F crit |
| Scaffold disc/well plate surface area | 8676.122015 | 1 | 8676.122 | 11.77456 | 0.002977 | 4.413873 |
| Cell seeding density | 1016.717587 | 2 | 508.3588 | 0.689905 | 0.514407 | 3.554557 |
| Interaction | 236.6088464 | 2 | 118.3044 | 0.160554 | 0.852878 | 3.554557 |
| Within | 13,263.35317 | 18 | 736.853 | – | – | – |
| Total | 23,192.80162 | 23 | – | – | – | – |
ANOVA: analysis of variance.
Cells in bold font indicate statistical significances.