Table 1.
SNP Site | Items | Sequence |
---|---|---|
rs361525 | Forward primer | CTCGGTTTCTTCTCCATCGC |
Reverse primer | CAGCCTCCAGGGTCCTACAC | |
Wild probe | TET-TCCCCATCCTCCCTGCTCtG ATT-BHQ | |
Mutation probe | FAM-CCCATCCTCCCTGCTCcG ATT-BHQ | |
rs1800629 | Forward primer | TTAGAAGGAAACAGACCACAG ACCT |
Reverse primer | GTAGGACCCTGGAGGCTGAAC | |
Wild probe | TET-TAGGTTTTGAGGGGCATGa GGACG-BHQ | |
Mutation probe | FAM-TAGGTTTTGAGGGGCATGg GGAC-BHQ | |
rs1800750 | Forward primer | CTTTCTGAAGCCCCTCCCA |
Reverse primer | TGCCCCTCAAAACCTATTGC | |
Wild probe | TET-CCTGCATCCTGTC TGGAAaTTAGAAGGA-BHQ | |
Mutation probe | FAM-CCTGCATCCTGTC TGGAAgTTAGAAGG-BHQ | |
rs1799964 | Forward primer | CATTCCTCAGAGCCGCTACAT |
Reverse primer | GGGATATGTGATGGACTCACCAG | |
Wild probe | TET-CTCCAGACCCTG ACTTTTCCTTCgTC-BHQ | |
Mutation probe | FAM-CCTCCAGACCCTGA CTTTTCCTTCaTC-BHQ | |
rs673 | forward primer | GGGTCCTACACACAAATCAGTC AGT |
Reverse primer | CCCTCACACTCCCCATCCT | |
Wild probe | TET-AAGACCCCCCTCgGA-MGB | |
Mutation probe | FAM-AAGACCCCCCTCaGA-MGB |
Abbreviation: SNP, single nucleotide polymorphisms.