Figure 3.

Microribonucleic acid-451 sensitizes prostate cancer cells to DTX in vitro.

(a) qRT-PCR detection of miR-451 expression in prostate cancer cells transfected with pcDNA/miR-NC (miR-NC) or pcDNA/miR-451 (miR-451); U6 was used as an internal control; (b) a CCK-8 assay was performed to detect the IC₅₀ values of DTX in pcDNA/miR-NC (or pcDNA/miR-451)-transfected prostate cancer cells; (c) flow cytometric analysis of early apoptosis rate of pcDNA/miR-NC (or pcDNA/miR-451)-transfected prostate cancer cells treated without (or with) DTX (5μg/l); (d) and (e) western blotting was used to detect cleaved caspase-3 (C-caspase-3) and caspase-3 in pcDNA/miR-NC (or pcDNA/miR-451)-transfected prostate cancer cells treated without (or with) DTX (5μg/l); β-actin was used as an internal control; the data represent the average of three independent experiments (means ± standard deviation), ^**p < 0.01.

DTX, docetaxel; miR-451, microribonucleic acid-451; pcDNA, plasmid complementory DNA; miR-NC, microRNA-negative control.