Fig. 5. Aggregation of AS(WT), AS(1-119), AS(1-103) in polystyrene plates at different pH values. (a–c) Change in the ThT fluorescence when 10 μM monomeric AS(WT) (a), AS(1-119) (b) or AS(1-103) (c) were incubated in polystyrene plates (using 20 μM ThT) at pH values ranging from 2 to 8 and 37 °C. (d) Variation in thalf of the reaction of amyloid formation as a function of pH for AS(WT) (green), AS(1-119) (blue) and AS(1-103) (red). (e) pH range where secondary nucleation was found to contribute to the aggregation process. The estimated net charges of the different peptides at the boundaries of the pH range are indicated.
