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. 2018 Jul 16;37:156. doi: 10.1186/s13046-018-0828-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Knockdown of JNK1 attenuated BMP4-activated autophagy and HCC proliferation: a Transfection efficiency of siRNA targeting JNK1 in HCC cells was confirmed by Western blot. b The expression level of LC3-II, p62 and BECN1 were detected by Western blot. Knockdown of JNK1 decreased BMP4-promoted LC3-II conversion as compared with the BMP4-treated group. c The expression levels of LC3-II were quantified by Image lab software by densitometric analysis and were normalized to the control groups. GAPDH was used as the internal control. n = 3, one-way ANOVA with post-hoc Tukey’s test. d Transfected cells were treated with BMP4 for 24 h. Cell viability was determined by CCK-8. n = 3, one-way ANOVA with post-hoc Tukey’s test