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. 2018 Jul 16;24:36. doi: 10.1186/s10020-018-0034-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — MiR-21-5p highly expressed in COAD tissues and cells. a The heat map showed that miR-21-5p was high expressed in COAD tissues compared with normal tissues. b The relative expression of miR-21-5p in cancer tissues was much higher than that in adjacent tissues detected by qRT-PCR. ^***P < 0.001, compared with adjacent tissue. c The expression of miR-21-5p in stage III-IV was notably higher than that in stage I-II detected by qRT-PCR. ^***P < 0.001, compared with stage I-II. d The expression of miR-21-5p in COAD cell lines (CW-2, T84, SW116, LoVo) was higher than that in normal colonic epithelial cell line (NCM-460) detected by qRT-PCR, which reached the highest level in LoVo cell line. ^**P < 0.01, ^***P < 0.001, compared with NCM-460