Fig. 6.

MiR-21-5p inhibited the expression of CHL1. a The heat map showed that the expression of CHL1 in COAD tissues was lower than that in normal tissues. b TargetScan predicted that there was a binding site for miR-21-5p in the 3’UTR of CHL1. The result of dual-luciferase reporter assay showed that the luciferase activity in miR-21-5p mimics and CHL1 3’UTR-wt co-combination group was lower than that in NC mimics and CHL1 3’UTR-wt co-combination group. In the meantime, there was no significant difference between miR-21-5p mimics and CHL1 3’UTR-mut co-combination group NC mimics and CHL1 3’UTR-mut co-combination group. c, d CHL1 expression in miR-21-5p mimics group was much lower than that in NC mimics group, while that in miR-21-5p inhibitor group was higher than that in NC inhibitor group. ^**P < 0.01, ^***P < 0.001, compared with NC mimics group, ^&&P < 0.01, compared with NC inhibitor group